Simultaneous detection of infectious human echoviruses and adenoviruses by an in situ nuclease-resistant molecular beacon-based assay.
نویسندگان
چکیده
A multiplex methodology using two nuclease-resistant molecular beacons that target specific genomic regions of adenovirus 2 and echovirus 17 during simultaneous infection in A549 cells is presented. Using fluorescence microscopy, visualization of enteroviral and adenoviral replication was possible within 3 h postinfection.
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 78 5 شماره
صفحات -
تاریخ انتشار 2012